Keywords

Rice Residue Protein Concentrate, Solubilization, Alkaline Protease

Abstract

The solubilization mechanism of insoluble rice residue protein concentrate (RDPC) was discussed from the perspective of enzyme reaction kinetics, including: a part of the dissolved protease adsorbs the insoluble rice residue protein concentrate (RDPC) to solubilize the insoluble protein, and the other part of the dissolved protease It is used to break the fragments dissolved in the solution after solubilization of insoluble proteins; the inhibition of enzyme activity by reaction products; the solubilization of additional substrates shows that there is a large amount of dissolved free enzyme in the reaction system that has not been fully utilized, and it also prompts The method of immobilized enzyme can be used to reduce the inhibition of enzyme activity by the product (low molecular weight peptide); the initial rate equation of the kinetic reaction is: V0=k2X1mK1[E]0[S]0/1+K1[E]0 +K1[S]0X1m=0.8859¡Á[E]0[S]0V0/1+6.3027[E]0+0.03590[S]0 The gel filtration of the hydrolyzate showed that alcalase degraded the insoluble components ( Pr) is likely to fit the “ZipperReaction” model

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Original research done by Liu Ji, Yao Huiyuan, Chen Zhengxing

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