Keywords
Rice Protein Angiotensin-Converting Enzyme Bioactive Peptides In Vitro Digestion Molecular Weight Distributionrice Protein Angiotensin-Converting Enzyme Bioactive Peptides In Vitro Digestion Molecular Weight Distribution
Abstract
In order to investigate whether the four rice proteins can produce ACE inhibitory active peptides and their activity status after simulated in vitro digestion, this study used rice as raw material and extracted the four rice proteins by the Osborne method. The gastrointestinal digestion process is simulated in vitro to study the production and activity of ACE inhibitory active peptides from digestive enzymatic hydrolysis, and at the same time detect the hydrolysis degree and molecular weight distribution of the enzymatic hydrolysis products. The experimental results showed that after the four rice proteins were digested by pepsin for 30 minutes, the ACE inhibitory activity of the enzymatic hydrolyzate products reached a high level. Subsequently, after the action of trypsin, the ACE inhibitory activity of the enzymatic hydrolyzate products decreased. The half inhibitory concentration IC50 values of 4 h digestion products of rice albumin, globulin, gliadin and glutenin are 1.45 mg/mL, 0.91 mg/mL, 1.19 mg/mL and 0.75 mg/mL respectively, and the molecular weight is concentrated at 1000 u The following are ACE inhibitory active peptides that are easily absorbed by the human body. At the same time, the unenzymatically digested protein has almost no ACE inhibitory activity. The results show that the in vitro digestion enzymatic hydrolysates of the four rice proteins have different ACE inhibitory activities. Among them, the digested products of rice gluten have the highest ACE inhibitory activity. Humans eat rice protein normally and gastrointestinal digestion can produce ACE inhibitory activity that is absorbed by the human body. Active peptides. In this study, the production of peptides with angiotensin-converting enzyme (ACE) inhibition activity was investigated from simulated in vitro digestion of four rice proteins, namely albumin, globulin, prolamin, and glutelin. The proteins were extracted from rice using the Osborne method . Production and ACE inhibiting activity, the degree of hydrolysis, and the molecular weight distribution of the proteolysis products were investigated. The results revealed that ACE inhibition activity of individual products obtained after proteolysis of the proteins, was high after 30 min of pepsin digestion. The activity decreased upon subsequent digestion with trypsin. The hydrolysates of rice albumin, globulin, prolamin, and glutelin after 4-hour digestion showed half-inhibition concentration (IC50) of 1.45, 0.91, 1.19, and 0.75 mg/mL, respectively, and their molecular weight was < 1000 u, which means they can be easily absorbed by the human body. At the same time, undigested proteins had almost no ACE inhibition activity. The result also showed the products of in vitro protein proteolysis had different ACE inhibition activities and that rice glutelin proteolysis product showed the highest ACE inhibition activity. Therefore, the gastrointestinal digestion of regularly consumed rice can produce ACE-inhibiting peptides that can be absorbed by the human body.
For further details regarding this article and its research, please do not hesitate to contact our team for assistance.
Original research done by Chen Qianqian
About ETprotein
ETprotein, a reputable plant protein vegan protein Chinese
factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include
Organic rice protein,
clear rice protein,
pea protein,
clear pea protein,
watermelon seed protein,
pumpkin seed protein,
sunflower seed protein,
mung bean protein,
peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.
Featured Products on Sale
Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price
Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife
Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price
Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife
Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market
We’re excited to provide you with top-notch proteins and great customer service!
