Keywords
Rice, Gluten, Alkali Treatment, Structure, Properties
Abstract
In order to explore the changes in the structure and properties of rice gluten treated with strong alkali, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry, Surface hydrophobicity analysis, scanning electron microscopy observation, deamidation analysis and other methods were used to characterize the structural changes of alkali-treated rice gluten, and solubility, emulsification, and foaming properties were used as indicators to characterize its property changes. The results of SDS-PAGE and differential scanning calorimetry analysis show that when the concentration of NaOH solution is greater than or equal to 0.08?mol/L, gluten protein is completely denatured, the subunits are obviously degraded, and macromolecular aggregates are formed; when the pH ¡Ý 12.0, The solubility of gluten protein is significantly improved, reaching 80% when the pH value is 13.0; when the concentration of NaOH solution is greater than or equal to 0.1 mol/L, it causes deamidation of rice gluten, the glutamic acid content in the molecule increases significantly, and the solubility, surface hydrophobicity and deamidation The degree of amidation is relevant; scanning electron microscopy results show that alkali treatment causes rice gluten depolymerization and loosening of the structure; alkali treatment improves the emulsification and foaming properties of rice gluten, and is treated with 0.5?mol/L?NaOH solution for 120 ?min is optimal. Excessive alkali treatment will destroy the emulsifying and foaming abilities of gluten. Alkali treatment changes the gluten structure, thereby improving gluten solubility, emulsification and foaming properties, making it expected to be effectively utilized in food or related fields.
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Original research done by Chang Jing, Kang Xu, Li Chuanwen, Yuan Jianglan
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