Analysis On The Composition Of High Molecular Weight Glutenin Subunits And Low Molecular Weight Glutenin Subunits Of Chinese Wheat Varieties
Keywords
Wheat Varieties
Abstract
In order to provide scientific basis for the selection and improvement of high-quality wheat varieties in my country, the composition and distribution of HMW-GS and LMW-GS of 235 popularized varieties and advanced lines in my country were analyzed using SDS-PAGE method. The results showed that HMW-GS and LMW-GS had 39 and 40 band pattern combinations respectively; a total of 34 alleles were found at 5 loci. Glu-A1, Glu-B1 and Glu-D1 have 3, 8 and 6 mutation sites respectively; subunit 1, Null, 7+9, 5+10 and 2+12 are the main HMW-GS types, with frequencies of 46.2%, 46.2%, 44.5%, 47.0% and 48.3%. Glu-A3 and Glu-B3 sites (Glu-D3 is not involved in this article) have 6 and 11 mutation sites respectively. Glu-A3c, Glu-A3d, Glu-B3e and Glu-B3j are the main LMW-GS types, with frequencies of 55.1%, 21.6%, 28% and 28.8% respectively. This study also found that at the Glu-D1 site of varieties Z75 and Mianyang 96-319, the subunit combination form is 5+12; at the Glu-D1 site of variety Zhou 92034, the subunit combination form is 2+10. Although the frequency of these two combined forms in this study was low, these two subunit combinations rarely appeared in previous studies.
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Original research done by Wu Fang, Dong Hui, Han Zhaoxue, Pan Zhifen, Deng Guangbing, Yu Maoqun
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