Keywords

Radionuclide Imaging Of Biodistribution Of 99M Technetium Merlotate In Mice Using Zheng’S Plant Protein

Abstract

Objective To explore the biological distribution characteristics and imaging characteristics of 99Tcm-ZPPAb in tumor-bearing mice. Methods ¢Ù 48 H22 liver cancer-bearing mouse models were established and divided into experimental group and control group, 24 mice in each group. Prepare 99Tcm-ZPPAb and 99Tcm-IgG. ¢Ú In vivo distribution experiment: 21 mice from each experimental group and 21 mice from the control group were injected with 99Tcm-ZPPAb and 99Tcm-IgG 7.4 MBq via the tail vein respectively at 30 min, 1, 2, 3, 4, 5, and 6 hours. Three animals were selected at this time, and the radioactivity uptake ratio per gram of tissue in each organ (%ID/g value) and the radioactivity count ratio between tumor and contralateral muscle tissue (T/NT value) were calculated. ¢Û Imaging experiment: Take 3 mice from each experimental group and 3 mice from the control group, inject 99Tcm-ZPPAb and 99Tcm-IgG 7.4MBq respectively through the tail vein, and calculate T at 30 min, 1, 2, 3, 4, 5, and 6 h. /NT value. Results: The labeling rate of 99Tcm-ZPPAb was 70%, and the labeling rate of 99Tcm-IgG was 85%. The results of the in vivo distribution experiment showed that the %ID/g value of the tumor tissue in the experimental group of tumor-bearing mice was significantly different between different phases (F=0.89, P=0.5231), and the %ID/g value of the tumor tissue at each phase was higher than Other tissues and organs except liver, kidney and blood (F=9.36, P=0.0007). There was a statistically significant difference in the %ID/g value of tumor tissue in the same phase between the two groups (F=13.49, P=0.0001). The difference in T/NT values in different phases in the experimental group was statistically significant (F=1.53, P=0.2237); the difference in T/NT values in the same phase between the two groups was statistically significant (F=23.69, P=0.0001). The results of the imaging experiment showed that the difference in T/NT values in different phases in the experimental group was statistically significant (F=1.00, P=0.4526), and the difference in T/NT values in the same phase between the two groups was statistically significant (F=6.09, P= 0.0048). Conclusion 99Tcm-ZPPAb is simple to label, has a high labeling rate, has high uptake and retention in tumor tissues, and is expected to become a positive tumor imaging agent

For further details regarding this article and its research, please do not hesitate to contact our team for assistance.

Original research done by Feng Jue, Zhang Wenjun, Zhang Xiaoming, Zheng Lichun

About ETprotein

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.

Featured Products on Sale

Organic Brown Rice Protein

Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price

Clear Rice Protein(Hydrolyzed Rice Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife

Pea Protein

Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price

Clear Pea Protein(Hydrolyzed Pea Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife

Organic Pumpkin Seed Protein

Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market

Contact sales@etprotein.com for a quotation and Free samples.

We’re excited to provide you with top-notch proteins and great customer service!