Keywords

Cultivated Wheat, Gliadin, Gene Cloning, Cd Toxicity, Evolutionary Relationship

Abstract

In order to comprehensively reveal the toxicity of Celiac disease (CD) induced by cultivated single-grain wheat and its application value in wheat quality improvement, 2~3 pairs of specific primers were designed based on the conserved sequences of registered genes, and AS-PCR technology was used to A new gliadin gene from E. aestivum was cloned, CD toxic peptide identified and homology analyzed. The results showed that 26 ¦Á- genes with unique coding regions were cloned from cultivated wheat (named: Gli-A-1~Gli-A-7, GenBank accession numbers are JN831382-JN831385 and JX828193~JX828195), ¦Ã- (named: Gli-R-1~Gli-R-6, GenBank accession numbers are HM120220, JX828376~JX828380), ¦Ø- (named: Gli-w-1~Gli-w-13) gliadin New gene and 1 known gene (Gli-R-7, ACJ03494). The identification analysis of CD toxic peptides shows that cultivated wheat has strong CD toxicity, which is distributed in 5 major T cell dominant peptides of gliadin and 4 “toxic peptides” except ¦Á-gliadin derived from the A genome. Except for the toxic peptides Glia-¦Á2 and Glia-¦Á, the other polypeptides are distributed in the proteins encoded by the cloned genes. Homology analysis of the cloned genes and known genes showed that the inferred amino acid sequences of ¦Á- and ¦Ã-gliadins have obvious differences in their genomic origins, while the differences in the genomic origins of the ¦Ø-gliadin gene are not obvious. In addition, the seven cloned ¦Á-gliadins have relatively wide variations, while the compositions of the ¦Ã- and ¦Ø-gliadin genes are relatively single and have extremely high similarity.

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Original research done by Li Yuge, Cui Yifei, Li Li, Li Suoping

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