Effects Of Whey Peptides On Inhibiting Oxidation And Improving Quality Of Pork Mince Subjected To Repeated Freezing And Thawing
Keywords
Whey Peptides, Antioxidant Effect, Repeated Freezing And Thawing, Minced Pork, Quality Characteristics
Abstract
In order to study the influence and mechanism of whey protein hydrolysates (WPH) on the oxidation and quality of repeatedly frozen and thawed pork mince, this experiment added different mass fractions (5%, 10%, 15%) of WPH to raw pork mince. Perform different freeze-thaw cycles (0 to 9) to detect the thawing loss of minced meat after freezing and thawing, thiobarbituric acid (thiobarbituric acid reactive substances, TBARS) value, and total volatile base nitrogen (TVB-N) value, textural properties, rheological modulus loss, protein carbonyl content and changes in Raman spectra. The results showed that without freezing and thawing, there was no significant difference between the indicators of the sample group and the blank group (P>0.05). After freeze-thaw treatment, the thawing loss rate of minced pork increased from 2.67% to 2.93% to 7.00% to 12.4%. Among them, 15% WPH significantly inhibited the increase in thawing loss (P<0.05) and improved the water retention of minced meat. When the freeze-thaw cycles reached 7 to 9 times, the 15% WPH group was better than the blank group and the unhydrolyzed whey peptide group in inhibiting the increase in TVB-N value, TBARS value and carbonyl content of minced meat (P<0.05). In terms of texture, with the increase in freezing and thawing times, the hardness, elasticity, cohesion and chewiness of minced pork showed a significant downward trend. After freezing and thawing 9 times, 15% WPH can increase the chewiness of minced pork from 9.63 g to 17 g; after freezing and thawing 5 times, the elasticity of the 15% WPH group reaches 0.69, exceeding butylatedhydroxyanisole (BHA). ) positive blank group; the hardness and cohesion of the WPH group and BHA group were significantly higher than the blank group; different amounts of WPH added improved the texture of minced pork, with the high-dose group having the best effect. Rheological results showed that the loss modulus G'' of pork mince in the WPH group decreased significantly. Raman spectroscopy showed that adding 15% WPH effectively inhibited the decrease in ¦Á-helix content and increase in ¦Â-sheet content of meat protein during freeze-thaw cycles, and protected the protein secondary structure. Experiments show that during the freeze-thaw cycle, WPH plays a role in inhibiting the oxidation of pork mince and improving product quality. For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Liu Yuan, Zhang Min, Peng Xinyan, Zeng Yulong, Wang Xiaoyu, He Hongjun, Shao Lingjian
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