Keywords

Amylin, Substance P, Rectum, Postnatal Development, Rabbit

Abstract

Objective To observe the expression and distribution characteristics of isletamyloid polypeptide (IAPP) and substance P (SP) in the rectum of rabbits at different developmental stages. Methods Ten rabbits each were collected at 5, 15, 25, 35, 60 and 90 days after birth. The rectal tissue was fixed in Bouin’s solution, dehydrated with graded alcohol, embedded in conventional paraffin, and made into serial sections. The location and expression of IAPP and SP immunoreactive positive cells were observed using immunohistochemistry SABC method and image analysis method. Results A small number of scattered IAPP-positive cells were seen in the rectum of rabbits on the 5th day after birth, mostly located in the epithelium. The number of IAPP-positive cells showed an obvious upward trend on the 35th day, reaching the maximum on the 60th day (P<0.05), and then decreased on the 90th day (P<0.05). Mainly Distributed in the lamina propria. Image analysis showed that the average gray value of IAPP-positive cells gradually decreased with the growth and development of rabbits, reaching the lowest on 60 days (P<0.05). SP-positive cells began to appear on the 15th day after birth, and the number of cells increased significantly after the 35th day (P<0.05), reaching the highest level on the 60th day (P<0.05). The average gray value also began to decrease on the 35th day (P<0.05) and reached the lowest level on the 60th day (P<0.05). Conclusion The expression of IAPP and SP in the rectum gradually changes with the growth and development of rabbits. It is speculated that they are involved in regulating the maturation process of rabbit rectal digestive function during postnatal development.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Hong Yan, Liang Wenmei, Han Jing, Hu, Xia Baijuan, Li Yixin  

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