Keywords

Flaxseed Cyclic Peptide Mixture, Antifungal Activity, Paecilomyces Vivarini, Aspergillus Aflatoxin, Thermal Stability

Abstract

Abstract Flaxseed cyclic peptide is isolated from flaxseed, but its biological activity is not yet clear. This article studied the inhibitory effect of flaxseed cyclolinopeptides (FCLPs) on mold. Paecilomyces variotii (AS 3.776) and Aspergillus flavus (CICC 2476) were selected as test strains, and a certain concentration gradient of FCLPs was added to potato dextrose agar (PDA) before and after high temperature sterilization at 121¡ãC. , plate spot method was used to inoculate and culture, and the colony diameter, spore number and spore germination rate were measured. The results show that the half-inhibition concentration (IC50) of FCLPs on Paecilomyces variotii spore formation is 0.53 mg/mL (high temperature sterilization) and 0.34 mg/mL (without high temperature sterilization) respectively; against Aspergillus flavus The IC50 values for sporulation were 0.31 mg/mL (high temperature sterilization) and 0.24 mg/mL (non-high temperature sterilization). After high-temperature sterilization, FCLPs maintained considerable antibacterial activity, with the lowest inhibitory activity against Paecilomyces variotii remaining at 76.8% (colony growth) and 76.7% (sporulation); for Aspergillus flavus, the lowest remaining inhibitory activity was 76.8% (colony growth) and 76.7% (sporulation), respectively. 61.6% (colony growth) and 41.7% (sporulation) remain. The average inhibition rate of spore germination rate by FCLPs was 17.8% (Paecilomyces variotii) and 7.8% (Aspergillus flavus). High performance liquid chromatography (HPLC) analyzed FCLPs extracted from the culture medium after mold cultivation and found that high-temperature sterilization reduced the content of cyclic peptides, which may be one of the reasons for the decrease in the antibacterial activity of cyclic peptides. However, the proportions of the four main cyclic peptides in the cyclic peptides did not change significantly, and no mold metabolites of cyclic peptides were found. In summary, FCLPs have certain inhibitory activity against mold, are relatively stable to high temperature processing, and can withstand the thermal processing of food. They have unique advantages over existing antimicrobial peptides.

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Original research done by 10.13995/j.cnki.11-1802/ts.014230

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