Keywords

Self-Assembling Peptides, Hydrogels, Nanofibers, Scaffold Materials, Tissue Engineering

Abstract

Objective To explore the effect of functionalized self-assembled nanopolypeptide DGEAmx hydrogel on the adhesion, proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (MSCs). Methods Atomic force microscopy was used to observe the nanoscale structural features formed by functionalized self-assembled peptides; adherent cells were photographed and counted using an inverted microscope to compare cell adhesion; cell proliferation was detected using the CCK-8 method; cells were observed on the surface of the material using a laser confocal microscope Morphology; detect alkaline phosphatase (ALP) activity, osteogenic differentiation-specific gene type I collagen (ICAM-1) and osteocalcin (OCN) expression to evaluate the osteogenic effect. Results The functionalized self-assembled peptide DGEAmx can form a nanofiber network structure according to the atomic force microscope; the results of photographing and counting adherent cells under an inverted microscope showed that there was a statistically significant difference in the number of adherent cells between the DGEAmx group and the RADA16-¢ñ group at 30, 60, and 90 minutes. Significance (P<0.05); CCK-8 method test results showed that on the 3rd, 5th, and 7th days of culture, the proliferation of MSCs in the DGEAmx group was significantly higher than that in the RADA16-¢ñ group (P<0.05); osteoinduction in the DGEAmx group was 3, 7, and 14 days. After 3, 7, and 14 days of osteogenic induction, the ALP activity was higher than that of the RADA16-¢ñ group (P<0.05); the Real-time PCR results showed that the DGEAmx group had higher expression of ICAM-1 and OCN than the RADA16-¢ñ group after 3, 7, and 14 days of osteogenic induction (P <0.05). Conclusion Functionalized self-assembled peptide DGEAmx nanofiber hydrogel scaffold can promote MSCs adhesion, proliferation and osteogenic differentiation, and has good application potential as a bone tissue engineering scaffold material.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Liu Jian, Hou Tianyong, Li Zhiqiang, Chen Beike, Deng Moyuan, Luo Fei, Xu Jianzhong  

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