Keywords

Immobilized Protease Hydrolysis Wastewater

Abstract

Adsorption methods were used to immobilize proteases on activated carbon, macroporous resin and silica gel carriers, and the immobilization effects, enzyme activities and protein hydrolysis effects of the three carrier proteases in starch processing yellow slurry wastewater were studied respectively. The batch test results showed that 3 The protease fixation effects of the three carriers are: silica gel 20.31%, macroporous resin 37.85%, activated carbon 61.95%; the pH when the enzyme activity is the highest is 5.5; the enzyme activities are 3.6mg/min, 21.48mg/min and 18.68mg/min respectively. After hydrolysis, the total amino acid content of the hydrolyzate of macroporous resin and activated carbon-immobilized enzyme increased 21.26 times and 14.6 times respectively compared with before hydrolysis; 16 kinds of amino acids were detected in the product, 2 kinds more than before hydrolysis. Constructed using macroporous resin as a carrier The immobilized enzyme reactor was tested for continuous water inflow. The results showed that the protein concentration in the incoming water significantly affected the hydrolysis effect of the reactor. When the protein concentration matched the enzyme concentration of the reactor (500mg/L), the reactor could obtain 80 % protein hydrolysis rate and 36.1% organic matter hydrolysis acidification rate. The volatile hydrolysis products are mainly composed of ethanol and acetic acid, accounting for 50.3% and 33.3% respectively. It is ethanol type hydrolysis

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Original research done by Yu Hongbing, Liu Jili, Zhang Lanying, Zhang Lei, Yang Xuemei, Lin Xueyu

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