Keywords

Pleurotus Eryngii Mycelium Polypeptides Immunological Activities Antitumor Activities

Abstract

In order to study the immune activity and anti-tumor effect of Pleurotus eryngii mycelium polypeptides, Pleurotus eryngii mycelium polypeptides were used as raw materials to test the survival rate of mouse macrophage Ana-1 cells, cytokines TNF-¦Á and IL- 6. The secretion amount, membrane surface protein TLR2 and TLR4 expression, phagocytic neutral red activity, and the ability to stimulate macrophages to produce NO and H2O2 were measured to study the immune activity of Pleurotus eryngii mycelium polypeptide; through the detection of human breast cancer cells BT -549 cells, cervical cancer cell Hela-229 and gastric cancer cell HGC-27 cells to study the anti-tumor effect of Pleurotus eryngii mycelium polypeptide. Experiments show that when the peptide concentration is in the range of 0.05~2 mg/mL, it can promote the proliferation of macrophages to a certain extent, increase the expression of membrane surface proteins TLR2 and TLR4, and increase the secretion of cytokines TNF-¦Á and IL-6. Enhances the ability of macrophages to phagocytose neutral red activity, and promotes macrophages to secrete NO and H2O2; it has a strong inhibitory effect on human breast cancer cells, cervical cancer cells and gastric cancer cells, indicating that Pleurotus eryngii Silk body peptides have good immune activity and anti-tumor activity, and the activity and concentration show a dose-effect relationship. The aim of the present work was to study the immunological and antitumor activities of polypeptides from Pleurotus eryngii mycelium, which was used as the raw material. The immunological activities of the polypeptides were investigated by measuring neutral red uptake capacity, the ability to stimulate macrophages to secret nitric oxide and hydrogen peroxide, the levels of the secreted cytokines tumor necrosis factor-¦Á and interleukin-6, the expression levels of the membrane surface proteins toll-like receptor (TLR)2 and TLR4, and Ana-1 cell survival rate. The antitumor activities of the polypeptides were studied through the inhibition of the proliferation of human breast cancer cells (BT-549), cervical cancer cells (Hela-229), and human gastric cancer cells (HGC-27). The results showed that within a concentration range of 0.05~2 mg/mL, the Pleurotus eryngii mycelium polypeptide could, to some extent, promote the proliferation of macrophages, enhance TLR2 and TLR4 expression levels and macrophage neutral red uptake, and increase the secretion of nitric oxide and hydrogen peroxide by macrophages. The polypeptide had strong inhibitory effects on the proliferation of the breast cancer, cervical cancer, and human gastric cancer cells. These results indicate that the polypeptides from Pleurotus eryngii mycelium had good immunological and antitumor activities with a concentration-response relationship.

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Original research done by Sun Yanan

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