Keywords

Enzymatic Hydrolysis, Clam Peptide, Raw264.7 Macrophage, Immunomodulatory Effect

Abstract

The immunomodulatory effect of Cyclina sinensis peptides (CSP) prepared by enzymatic hydrolysis on RAW264.7 macrophages was studied. Using the relative proliferation rate of RAW264.7 macrophages as an indicator, pepsin was used to enzymatically hydrolyze green clam meat. After ultrafiltration and freeze-drying of the enzymatic solution, the methyl thiazolyltetrazolium (MTT) method was used to screen out the ones with the highest proliferation activity. The components were studied for their immunomodulatory effects. MTT cell proliferation assay, inverted microscope observation, neutral red phagocytosis assay, nitrate reductase method and enzyme-linked immunosorbent assay (ELISA) were used to determine the growth of CSP-5 on RAW264.7 macrophages. It is related to proliferation, cell morphology, phagocytosis, nitric oxide (NO) secretion ability and cytokines interleukin (IL)-1¦Â, IL-6 and tumor necrosis factor-¦Á (TNF- ¦Á) The effect of secretion ability; the effect of CSP-5 on the distribution of macrophages in each cycle was also studied. The results show that CSP-5 with a molecular mass less than 3 kDa has a significant promoting effect on the growth and proliferation of RAW264.7 cells, and mainly acts in the G0/G1 phase, affecting the cell phagocytosis, NO secretion and cytokine secretion abilities. The increase has a significant promoting effect; morphological observation results show that after the action of CSP-5, the size of macrophages becomes larger and more pseudopods grow. It can be seen that CSP-5 has the potential role of activating macrophages and enhancing the body’s immunity.

For further details regarding this article and its research, please do not hesitate to contact our team for assistance.

Original research done by Ding Guofang, Yu Fangmiao, Ye Shengwang, Tang Yunping, Zhang Xiaojun, Li Wei, Yang Zuisu, Huang Fangfang

About ETprotein

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.

Featured Products on Sale

Organic Brown Rice Protein

Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price

Clear Rice Protein(Hydrolyzed Rice Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife

Pea Protein

Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price

Clear Pea Protein(Hydrolyzed Pea Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife

Organic Pumpkin Seed Protein

Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market

Contact sales@etprotein.com for a quotation and Free samples.

We’re excited to provide you with top-notch proteins and great customer service!