Keywords

Newcastle Disease Virus, Matrix Protein, Transport Receptor Protein, Nuclear Localization

Abstract

Abstract: [Objective] To identify cellular proteins related to the nuclear import of Newcastle disease virus (NDV) matrix protein (M), in order to elucidate the molecular mechanism of nuclear localization of NDV M protein. [Method] The nuclear transport receptor protein KPNA1¨CKPNA6 and KPNB1 genes were cloned from chicken embryo fibroblasts, constructed into eukaryotic expression vectors, and co-transfected into HEK with the recombinant eukaryotic expression vector expressing NDV M protein. -293T cells, identification of nuclear transport receptor proteins that interact with NDV M protein through co-immunoprecipitation method. In addition, M protein was co-expressed with Ran protein mutants or nuclear transport receptor protein deletions that interacted with M protein, and the cellular proteins related to nuclear transport of M protein were determined through fluorescence co-localization. [Results] The constructed recombinant eukaryotic expression vector can be expressed correctly in HEK-293T cells; through indirect immunofluorescence observation, it was found that except for Myc-KPNA2 protein, which is located in the cytoplasm, other nuclear transport receptor proteins are associated with M protein. showed the same nuclear localization. Co-immunoprecipitation test results showed that M protein interacts with both KPNA1 protein and KPNB1 protein. Further fluorescence co-localization observation revealed that co-expression of M protein with KPNA1 protein deletion (DN-KPNA1) did not change the nuclear localization of M protein, but co-expression with KPNB1 protein deletion (DN-KPNB1) caused M protein to become cytoplasmic. The localization shows that the nuclear transport of M protein requires the participation of KPNB1 protein. In addition, M protein was co-expressed with the Ran protein mutant Ran-Q69L, and fluorescence observation showed that the M protein also changed from nuclear localization to cytoplasmic localization, indicating that the nuclear transport of M protein also requires the assistance of Ran protein. [Conclusion] KPNB1 and Ran protein jointly mediate the nuclear transport of NDV M protein. The process is that KPNB1 protein first interacts with the M protein and forms a complex, and then completes the nuclear transport through the assistance of Ran protein.

For further details regarding this article and its research, please do not hesitate to contact our team for assistance.

Original research done by Liu Xiufan, Ji Xinqin, Hu Shunlin, Xu Houqiang, Xu Haixu, Zhao Jiafu

About ETprotein

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.

Featured Products on Sale

Organic Brown Rice Protein

Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price

Clear Rice Protein(Hydrolyzed Rice Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife

Pea Protein

Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price

Clear Pea Protein(Hydrolyzed Pea Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife

Organic Pumpkin Seed Protein

Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market

Contact sales@etprotein.com for a quotation and Free samples.

We’re excited to provide you with top-notch proteins and great customer service!