Keywords

Somatostatin, Amylin, Postnatal Development, Gastric Antrum, Rabbit

Abstract

Objective: To observe the localization and expression of somatostatin (SS) and islet amyloid polypeptide (IAPP) in the gastric antrum mucosa of rabbits during postnatal development, and explore their relationship with the body’s digestive function. Methods: 10 rabbits each at 5, 15, 25, 35, 60 and 90 days after birth were sacrificed and the gastric antrum tissue was harvested, embedded in routine paraffin, and made into serial sections. Immunohistochemistry SABC method and image analysis were used to detect SS. , the location and expression of IAPP immunoreactive positive cells were analyzed. Results: SS and IAPP were expressed in the gastric antrum mucosa of rabbits at different developmental stages. The positive product is brown and located in the cytoplasm. On the 5th and 15th day after birth of rabbits, SS-positive cells were dispersed in the mucosal epithelium, and the staining was lighter. It gradually increased on the 35th day, with the largest number on the 60th day (P<0.05), and decreased to the 35d level on the 90th day; the average gray value of the cells It decreased at 35 days and reached the lowest at 60 days (P<0.05). Most IAPP-positive cells are located in the connective tissue of the gastric antrum mucosa. The number of IAPP-positive cells in rabbits gradually increases from the 5th day after birth, especially on the 60th day after birth (P<0.05); the average gray value decreases after the 35th day and is lower than 5, 15 and 25d groups (P<0.05). Conclusion: The expression of SS and IAPP in the gastric antral mucosa of rabbits changes with growth and development. They may participate in the digestive activities of the postpartum body in an endocrine and paracrine manner, and play a role in the growth, repair and self-protection of the gastric mucosa. Play an important role.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Xia Baijuan, Liang Wenmei, Hong Yan, Han Jing, Hu Yun, Li Yixin  

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