Keywords

Imitation Sea Cucumber Extract, Peptides, Antioxidant, Mice

Abstract

This experiment uses imitation sea cucumber essence as raw material to prepare peptides and examine their antioxidant activity in vitro and in vivo. Using the ability to scavenge hydroxyl radicals (?OH) as an indicator, the activity of the enzymatic hydrolysis products produced by five enzymatic hydrolysis methods of papain, complex protease and flavor protease was investigated to optimize the experimental enzymes. After ultrafiltration and fractionation, determine the molecular weight distribution, soluble protein content, peptide content and clearance of components at each level? OH ability. Select clear? For low molecular weight peptide components with good OH ability, determine the amino acid composition and set low, medium and high dose groups (100, 200, 600 mg/kg (based on body weight, the same below)), model control group, and The blank control group was subjected to intragastric administration of mice for 42 days. After the experiment was completed, the superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, glutathione (GSH) content, and Malondialdehyde (MDA) content and protein carbonyl content. The results show that <1 000 u japonicus japonicus polypeptide hydrolyzed by papain and prepared by ultrafiltration has good in vitro clearance? OH capacity, its half inhibitory concentration is 6.02 mg/mL, and the content ratio is 58%. The amino acid composition is rich in acidic amino acids such as glutamic acid and hydrophobic amino acids such as tyrosine. The mouse antioxidant model experiment showed that the experimental group grew normally, the SOD activity in the mice in the high-dose peptide group was significantly increased (P<0.05), the GSH content was significantly increased (P<0.01), and the GSH-Px activity was also dose-dependent. increased, but the difference was not significant (P>0.05). MDA and protein carbonyl content were effectively reduced, and the latter reached significant difference (P<0.05). Research results show that <1 000 u sea cucumber imitation polypeptide has the effect of improving the antioxidant capacity of mice and can be used as a raw material for the development of related functional products.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Liu Jingxi, Liu Shaowei, Liu Xin, Zhang Jian, Zhang Yi, Wang Gongming, Zhao Yunping  

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