Keywords

Egg White Peptide, Peptide Calcium Complex, Enzymatic Hydrolysis, Preparation

Abstract

In order to obtain a calcium supplement preparation with high biological potency and increase the added value of egg white protein, the egg white was first enzymatically modified, and experimental enzymes were screened. Orthogonal experiments were used to optimize the enzymatic hydrolysis conditions and peptide calcium reaction conditions. , and use infrared spectroscopy to compare the changes in the spectra before and after the reaction. The results show that: choose alkaline protease first and then neutral protease for double-enzyme enzymatic hydrolysis. The enzymatic hydrolysis conditions are substrate mass fraction 6%, alkaline enzyme bottom ratio 1:100, pH 8.0, enzymatic hydrolysis time 3 hours; neutral The enzyme-to-enzyme ratio is 1:25, the pH is 7.0, and the enzymatic hydrolysis time is 3 hours. The optimal reaction conditions for preparing peptide calcium complexes are peptide calcium (CaCl2) mass ratio 2.5:1, pH 9.5, substrate mass fraction 4%, and temperature 55¡ãC. Under these conditions, the soluble calcium content is 21.15 mg/L. The degree of hydrolysis is 8.66%. When preparing egg white peptides, the hydrolysis effect of double enzymes is better than that of a single enzyme. Egg white peptides and calcium form a double monodentate complex, with a calcium mass fraction of 8.75%.

For further details regarding this article and its research, please do not hesitate to contact our team for assistance.

Original research done by He Hui, Zhang Wenjun, Li Jiangtao, Han Ying, Ma Zhili

About ETprotein

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.

Featured Products on Sale

Organic Brown Rice Protein

Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price

Clear Rice Protein(Hydrolyzed Rice Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife

Pea Protein

Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price

Clear Pea Protein(Hydrolyzed Pea Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife

Organic Pumpkin Seed Protein

Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market

Contact sales@etprotein.com for a quotation and Free samples.

We’re excited to provide you with top-notch proteins and great customer service!