Keywords

C-Terminal Sequencing, Protein, Peptide, Carboxypeptidase Method, Chemical Method, Tandem Mass Spectrometry, Review

Abstract

The C-terminal sequence is an important structural and functional part of proteins and polypeptides, and even plays a decisive role in the biological functions of proteins. In addition, C-terminal cleavage is also one of the important post-translational modifications of proteins. With the continuous deepening of proteomics research, Protein C-terminal sequencing will play an increasingly important role in the study of its function. Some new protein C-terminal sequencing methods have been established, which have improved sensitivity and reproducibility and can be applied at the proteome level. This article systematically introduces protein C Three methods of C-terminal sequencing: the principles, characteristics, applications and new progress in proteomics research of carboxypeptidase method, chemical method and tandem mass spectrometry. With the continuous establishment of new C-terminal sequencing methods, it will be used in proteomics research play a greater role.

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Original research done by Gao Yanfei, Wang Hongxia

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