Keywords

Phosphopeptides, Enrichment, Sample Preparation

Abstract

Phosphorylation is a type of post-translational modification of proteins that exists widely in organisms and is involved in the regulation of most life activities. In view of the important biological significance of phosphorylation modification, related research has always been the focus of many scientific researchers. However, because phosphorylated proteins have characteristics such as wide distribution, low relative abundance, and continuous dynamic changes, the analysis process has problems such as high complexity, low efficiency, and difficulty. The development of efficient and stable sample preparation methods is one of the key factors to achieve high-throughput phosphorylation modification analysis. In addition to the classic enrichment materials based on metal oxide affinity chromatography and immobilized metal affinity chromatography, in the past decade, some methods have been used to separate phosphorylated peptides through ion exchange, hydrogen bonding, ligand exchange, hydrophobic interaction, etc. New materials are also gradually being applied. This article briefly describes the principles and research progress of these emerging enrichment methods. Phosphorylation is a type of protein post-translational modification widely existing in organisms, and it is involved in the regulation of most life activities. In view of its important biological significance, the relevant research has been the focus of many scientific researchers. However, due to the characteristics of wide distribution, low relative abundance and continuous dynamic changes of phosphorylated proteins, the analysis process is extremely complicated, inefficient and difficult. It is one of the key factors to achieve high-throughput phosphorylation analysis via developing efficient and stable sample preparation methods. In the past decade, in addition to the classic enrichment materials based on metal oxide affinity chromatography and immobilized metal affinity chromatography, several novel ones based on ion exchange, hydrogen bonding, ligand exchange, and/or hydrophobic interaction have been used to separate phosphopeptides. This review briefly describes the enrichment principles as well as the research progress of these emerging methods.

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Original research done by Luo Yinjian, Zhang Qiwei, Xiao Chen, Zheng Qi

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