Study On The Effect Of Avian Pancreatic Polypeptide On Apprmrna Expression In Liver And Small Intestine Tissues Of Broiler Chickens
Keywords
Avian Pancreatic Polypeptide, Avian Pancreatic Polypeptide Receptor, Broiler Chicken, Real-Time Fluorescence Quantitative Pcr
Abstract
This experiment aimed to study the effect of avian pancreatic polypeptide (APP) on the expression of APPRmRNA in the liver and small intestine tissues of broiler chickens. The experiment used APP crude extract and chromatographic APP products as materials, and selected 90 one-week-old Avian broiler chickens and randomly divided them into 5 groups, with 3 replicates in each group and 6 chickens in each replicate. ¢ñ was set as the control group, ¢ò and ¢ó were set as the feeding and drinking group, and ¢ô and ¢õ were set as the injection group. At the end of the 7th week, the liver and small intestine tissues were slaughtered and the SYBRGeen¢ñ real-time fluorescence quantitative PCR (RT-PCR) method was used to conduct relative quantitative analysis of APPRmRNA expression in the liver and small intestine. The results showed that the expression levels of APPRmRNA in the liver and small intestine of the injection group and the feed drink group showed a downward trend compared with the control group, and the degree of decrease in the injection group was greater than that of the feed drink group; among them, the expression levels of APPRmRNA in the injection group and the feed drink group were both The chromatographic APP product showed a more obvious decrease than the APP crude extract, suggesting that the addition of APP can inhibit the expression of APPRmRNA. The results of this study provide a certain theoretical basis for further exploring the relationship between APP and its receptors and elucidating its physiological functions and mechanisms.
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Original research done by 10.3969/j.ssn.1006-267x.2009.01.018
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