Keywords

Nbd Peptide, Nf-&Kappa, B, Ischemia-Reperfusion Injury, Liver Transplantation

Abstract

Objective To use the SD-SD rat orthotopic liver transplantation model to observe the effect of NBD peptide pretreated donor liver on postoperative liver ischemia-reperfusion injury (ischemiareperfusion injury, IRI), and to explore its possible protective mechanism. Methods 105 SD rats were randomly divided into 3 groups by lottery method, NBD pretreatment group (24 pairs of animals, donors were intraperitoneally injected with 8 mg/kg NBD peptide 2 hours before surgery and an orthotopic liver transplantation model was established), transplantation group ( 24 pairs of animals, injected with the same volume of normal saline as control) and sham operation group (9 animals). The animals were sacrificed 3, 6, and 24 hours after surgery, and the ALT levels in the three groups were detected, and the TNF-¦Á content in the serum was detected by ELISA. Liver tissues from the NBD pretreatment group and transplantation group were collected, and HE staining was used to observe liver pathological changes. TUNEL was used to detect hepatocyte apoptosis, EMSA was used to detect NF-¦ÊB transcriptional activity, and Westernblot was used to detect the expression of p-IKK-2 and I¦ÊB¦Á in liver tissue. Results The ALT levels and TNF-¦Á content at each postoperative time point in the NBD pretreatment group and transplantation group were significantly higher than those in the sham operation group. The ALT level, NF-¦ÊB activity, p-IKK-2 expression, and TNF-¦Á content in the NBD pretreatment group were significantly lower than those in the transplantation group (P<0.05); the I¦ÊB¦Á expression was significantly enhanced compared with the transplantation group (P<0.05); Six hours after surgery, the pathological damage and apoptotic cells in the NBD pretreatment group were significantly reduced compared with the transplantation group. Conclusion NBD peptide pretreatment of donors can effectively improve liver transplantation IRI by inhibiting NF-¦ÊB activity in donor livers.   For further details regarding this article and its research, please do not hesitate to contact our team for assistance. Original research done by Cheng Mingxiang, Li Qing, Liang Shaoyong, Hao Yonggang, Yuan Zhibin, Tu Bing, Liu Changan, Gong Jianping, You Haibo  

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