Keywords

Subcritical Water Ultrasound Extraction Spirulina Crude Protein Antioxidantsubcritical Water Ultrasound Extraction Spirulina Crude Protein Antioxidant

Abstract

This article uses spirulina powder as raw material and uses ultrasonic coupling subcritical water method to extract spirulina crude protein. The structure of the extraction kettle in the subcritical water extraction equipment was improved to increase the contact area between raw materials and subcritical water, and the protein extraction rate was significantly increased from 10.30% to 40.20% (p<0.01); the temperature, ultrasonic power, and The influence of factors such as pressure and time on the extraction rate was determined. The order of the four influencing factors was: temperature>ultrasonic power>extraction time>pressure. Through orthogonal experimental design and verification, the optimal method for extracting Spirulina crude protein was obtained. The optimal process conditions are temperature 150 ¡æ, ultrasonic power 200 W, extraction time 80 min, and pressure 10 MPa. Under these optimal process conditions, the extraction rate of crude protein is 74.32%; evaluated by ABTS, DPPH, and OH free radical scavenging rate methods The antioxidant capacity of Spirulina crude protein extracted at different temperatures showed that the crude protein had strong antioxidant activity in the concentration range of 0.15~10 mg/mL. In summary, the ultrasonic coupled subcritical water extraction technology has created a new technology with high efficiency, low consumption and environmental protection for the extraction of seaweed protein. In this work, ultrasound coupling subcritical water extraction of crude protein from Spirulina platensis was investigated. The extractive reactor was redesigned to increase the contact area of the raw material and subcritical water, and the extraction rate of crude protein was improved significantly from 10.30% to 40.20% (p<0.01); The factors affected on the extraction rate, including temperature, ultrasonic power, pressure and time were systematically discussed using single factor level analysis, and the order was temperature>ultrasonic power>extraction time>pressure; The optimal extraction conditions were obtained by orthogonal design and verification, and the best extraction rate of 74.32% was achieved under the following extraction conditions: temperature of 150 ¡æ, ultrasonic power of 200 W, extraction time of 80 min and pressure of 10 MPa. The antioxidant activity of crude protein extracted with different temperatures was determined by measuring its reducing power and clearance ability on DPPH and ABST+ and OH- radicals in vitro, and the results showed that crude protein extraction exhibited an excellent antioxidant activity in a dose-dependent manner at concentrations of 0.15~10.00 mg/mL. Therefore, ultrasound coupling subcritical water extraction of protein from marine algae can be used as a new technology with high efficiency, low energy consumption and environmental protection.

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Original research done by Hu Shuangfei

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