Keywords

Solid-State Fermentation, Composite Bacteria, Soybean Meal, Soybean Peptides, Response Surface Analysis

Abstract

Common feed soybean meal was used as raw material, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger were selected as fermentation strains. Soluble proteins (including free amino acids and small peptides) in the fermentation products were used as measurement indicators to study the factors affecting the production of soybean peptides from solid-state fermented soybean meal. The main factors, including different binary combination strains, strain ratio, strain inoculation amount, fermentation time, substrate moisture content, and fermentation temperature, were studied in a single factor; and the response surface method was used to evaluate the optimal combination of strains for solid-state fermentation of soybeans. Peptide conditions were optimized. The results show that the solid-state fermentation of soybean meal with a mixture of Bacillus subtilis and Aspergillus niger has the highest soybean peptide content; the optimal conditions are: inoculation volume 27.95% and 52.71%, fermentation temperature 35.92¡ãC, matrix moisture content 52.71%, Bacillus subtilis and Aspergillus niger. The strain ratio of Aspergillus is 2:1, the fermentation time is 48 hours, and the soybean peptide content under these optimized conditions is 8.73%.

For further details regarding this article and its research, please do not hesitate to contact our team for assistance.

Original research done by Qian Senhe, Li Rongyu, Wei Ming, Xue Zhenglian

About ETprotein

ETprotein, a reputable plant protein vegan protein Chinese factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality organic bulk vegan protein and plant proteins. They include Organic rice protein, clear rice protein, pea protein, clear pea protein, watermelon seed protein, pumpkin seed protein, sunflower seed protein, mung bean protein, peanut protein etc. Their offerings, characterized by a neutral taste, non-GMO, allergen-free attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETprotein specialization includes exporting and delivering tailor-made protein powder and finished nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, and Infant Formula, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETprotein reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email sales(at)ETprotein.com today.

Featured Products on Sale

Organic Brown Rice Protein

Protein: 80%, 85%,90%
NON-GMO, Solvents-free, Soy-free, Gluten-free, Low sodium, Low heavy metals, Pesticides free
Neutral taste, Lower fat, Longer shelf life
Organic COI/TC certificate is available for the European market
NOP import certificate is available for the USA/Canada market
Competitive price

Clear Rice Protein(Hydrolyzed Rice Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Instant solubility in water, generates a clear solution, no sediments, and Stable for a longer shelflife

Pea Protein

Protein: 80%, 85%,90%
Neutral taste, Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium,Lower heavy metals
Types for vegan meat, vegan beverage
Competitive price

Clear Pea Protein(Hydrolyzed Pea Protein)

Protein Min 85%
Neutral taste, win over all over the world’s existing competitors
Non-GMO, Solvents free, Soy-free, Gluten-free, Vegan, Low sodium, Lower heavy metals
Instant solubility in water, generates clear solution, no sediments, Stable for longer shelflife

Organic Pumpkin Seed Protein

Protein Min 70%
Neutral typical smooth taste, win over all over other existing competitors
NOP import certificate is available for the USA/Canada market

Contact sales@etprotein.com for a quotation and Free samples.

We’re excited to provide you with top-notch proteins and great customer service!