Keywords

Oyster Peptides, Training, Testosterone, Star Mrna Expression Oyster Peptide Training Testosterone Star Mrna Expression, Oyster Peptide Training Testosterone Star Mrna Expression, Oyster Peptide Training Testosterone Star Mrna Expression, Oyster Peptide Training Testosterone Star Mrna Expression

Abstract

In order to explore the effect of supplementing oyster peptide on the expression of blood testosterone, LH and StAR mRNA in rats undergoing long-term heavy load training. 32 male SD rats were randomly divided into 4 groups: control group (QC), simple exercise group (SE), exercise+ Low-dose oyster peptide group (E+LOP) and exercise + high-dose oyster peptide group (E+HOP). Except for the QC group, each group established a long-term heavy load training model according to the training plan every week. The E+LOP group and E+ HOP group The corresponding dose of oyster peptide was supplemented 2 hours after each exercise. The QC group and SE group were supplemented with an equal volume of normal saline, and the weight of rats in each group was recorded every weekend. After 5 weeks, tissue materials were taken under anesthesia, and blood was taken to measure hemoglobin (HGB). , Radioimmunoassay was used to detect the serum testosterone (T) and luteinizing hormone (LH) contents of rats in each group, and RT-PCR was used to detect the expression level of StAR mRNA in testicular tissue. The results showed that: after 5 weeks, compared with the QC group, the SE group had HGB , the serum T level decreased statistically significantly (P<0.05), the LH level had no statistically significant difference (P>0.05), and the weight gain of rats decreased with the increase of weeks (P<0.01) , StAR mRNA expression was low (P£¼0.01); compared with the SE group, the HGB and serum T levels in the E+ LOP and E+HOP groups were statistically significantly higher (P£¼0.05), while the LH level was not statistically significant. There was a significant difference (P£¾0.05), the weight of rats increased with increasing weeks (P£¼0.05), and the expression of StAR mRNA was high (P£¼0.05); there was no statistical difference between the E+LOP and E+HOP groups. There is a scientifically significant difference (P>0.05). Therefore, supplementing oyster peptides can promote the expression of StAR mRNA in testicular tissue, thereby increasing testosterone synthesis in interstitial cells, which is beneficial to improving hypotemia caused by long-term heavy-load training. To investigate the effect of oyster peptides supplementation on serum testoserone,LH content and StAR mRNA expression in rats during the long-term and high loading training.Thirty two male rats were randomly divided into four groups:QC,SE,E+LOP and E+HOP.All the groups except QC group followed a protocol of treadmill training,E+LOP and E+HOP groups ingested oyster peptides with the corresponding content after two hours,at the same time,QC and SE consumed physiological saline with the same volume,weight of rats were recorded on the weeken during the training. After 5-week training, all the rats were taken anesthesia for tissue sampling. Contents of HGB, T, and LH were determined, StAR mRNA expressions of the rats’ testicular tissue were detected by PT- PCR.Compared with the QC group After five weeks,HGB and serum T Contents of SE group significantly decreased(P<0.05),and StAR mRNA expressions of the rats' testicular tissue significantly decreased,change of the mean body weights became less with the week number increasing(P<0.05); Compared with the SE group,HGB and serum T Contents of E+LOP group and E+HOP group significantly increased(P<0.05),StAR mRNA expressions significantly increased(P<0.05),change of the mean body weights obviously became greater with the week number increasing(P<0.05).but there was no difference in contents of LH among four groups(P>0.05),and there was no difference in the above indicators between E+LOP group and E+HOP group(P>0.05).Oyster peptides supplementation can elevate StAR mRNA expressions of the rats’testicular tissue,and prompt the synthesis of testoserone,which improves symptoms of the low serum testoserone

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Original research done by Luo Qijun, Lu Shunbao, Li Hong, Jingzhou, Xiong Jingyu, Zhang Yanjie

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