Keywords

Gelsolin, Pollen, Plant Actin

Abstract

Using plant pollen as experimental material, the effect of animal-derived gelsolin on plant actin filaments and the localization of gelsolin in plant cells were studied. Experiments using centrifugal precipitation of filamentous actin and electron microscopy negative staining The results show that actin filaments in purified pollen and crude extracts can be cleaved by gelsolin, and this shearing depends on Ca2+. In addition, immunoprecipitation was used to determine gelsolin and pollen actin. Monomer binding experiments showed that when Ca2+ was present in the solution, the molar ratio of pollen actin to gelsolin was approximately 2.0¡À0.21; after removing Ca2+ with EGTA, the ratio decreased to 1.2¡À 0.23; after adding PIP2, this binding ratio dropped to 0.8¡À0.10, indicating that the binding between plant actin and gelsolin has properties similar to animal actin and is regulated by Ca2+ and PIP2. Pollen coagulation The results of immunoidentification and immunofluorescence localization experiments of gelsolin showed that gelsolin exists in pollen. In ungerminated pollen grains, gelsolin is mainly concentrated in the germination furrow, while in pollen tubes that have germinated for 2 hours, the cytoplasm of the pollen tube Fluorescence distribution can be seen inside the pollen tube, but the fluorescence at the top of the pollen tube is stronger. It is speculated that gelsolin may be involved in pollen germination and pollen tube growth.

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Original research done by Tao Zhihua,Ren Haiyun

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